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4oc  (Atlas Antibodies)


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    Structured Review

    Atlas Antibodies 4oc
    4oc, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4oc/Anti-GAD1/pm41554730-306-51-71
    Average 90 stars, based on 2 article reviews
    4oc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer’s disease gene expression linked to neuropathology
    Article Snippet: .. Antibodies were incubated overnight at 4oC with end-over-end rotation: anti-CREB3L2 (2 μg; HPA015068, Atlas Antibodies) or, as negative control, rabbit normal serum IgG (#2729). .. Immunoprecipitates were captured using Protein G magnetic beads (#9006, Cell Signaling Technology) and washed with low- and high-salt buffers.

    Article Title: Central amygdala single-nucleus atlas reveals chromatin and gene transcription dynamics in human alcohol use disorder.
    Article Snippet: .. Following washes in phosphate buffer, tissue sections were pretreated for antigen retrieval (0.01 M sodium citrate for 45 min at 900C), followed sequentially by incubation in 1% sodium borohydride (30 min), PB wash, incubation in 0.3% Triton X-100 (30 min), blocked in 20% normal donkey serum (1.5 hr), and incubated at 4oC for 72 hr with the following primary antibodies: goat anti-Gad1 1:100 dilution (R&D Systems AF2086), rabbit anti-PENK 1:500 dilution (Atlas Antibodies HPA013138), anti-Rb 647; rabbit anti- FREM1 1:50 dilution (Proteintech 13086-1-AP). .. Secondary antibody incubations were overnight at 4oC with anti-goat Alexa fluor-488 or anti-Rb Alexa Fluor- 647 (Invitrogen, Life Technologies, Carlsbad, CA).

    Article Title: CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer’s disease gene expression linked to neuropathology
    Article Snippet: .. Approximately 6 μg of chromatin, diluted in 400 μl of ChIP buffer, was used per immunoprecipitation; CREB3L2-bound DNA was immunoprecipitated by overnight incubation at 4oC with anti-CREB3L2 serum (2 μg; HPA015068, Atlas Antibodies). ..

    Negative Control:

    Article Title: CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer’s disease gene expression linked to neuropathology
    Article Snippet: .. Antibodies were incubated overnight at 4oC with end-over-end rotation: anti-CREB3L2 (2 μg; HPA015068, Atlas Antibodies) or, as negative control, rabbit normal serum IgG (#2729). .. Immunoprecipitates were captured using Protein G magnetic beads (#9006, Cell Signaling Technology) and washed with low- and high-salt buffers.

    Chromatin Immunoprecipitation:

    Article Title: CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer’s disease gene expression linked to neuropathology
    Article Snippet: .. Approximately 6 μg of chromatin, diluted in 400 μl of ChIP buffer, was used per immunoprecipitation; CREB3L2-bound DNA was immunoprecipitated by overnight incubation at 4oC with anti-CREB3L2 serum (2 μg; HPA015068, Atlas Antibodies). ..

    Immunoprecipitation:

    Article Title: CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer’s disease gene expression linked to neuropathology
    Article Snippet: .. Approximately 6 μg of chromatin, diluted in 400 μl of ChIP buffer, was used per immunoprecipitation; CREB3L2-bound DNA was immunoprecipitated by overnight incubation at 4oC with anti-CREB3L2 serum (2 μg; HPA015068, Atlas Antibodies). ..



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